Staff profile for Dr Peregrine Osborne

Position

Senior Research Officer, Group Leader

Job description

Research Scientist

Organisation

Research Scientist, University of Sydney

Qualifications

Education

BSc(Hons), PhD: University of Melbourne

Research interests

Humans often experience long periods of pain as an unwanted legacy of  injury and disease. Unfortunately this chronic pain continues even when there is complete recovery from the underlying condition that triggered the pain in the first place. Our group studies biological mechanisms in the nervous system that either sustain or intensify pain for long periods, or reduce the pain-relieving properties of opioids and other analgesic drugs. We are especially interested in how chronic pain is caused by neuronal plasticity associated with pathological changes in the functioning or structure of nerve cells in pain circuits.

We are currently engaged in non-human research investigating 1) pain in the context of spinal cord and peripheral nerve injury, 2) pelvic pain and the role of circulating estrogens, 3) opioid-induced hyperalgesia, and 4) mechanisms of opioid receptor desensitization. Pain research is inherently multidisciplinary and this is reflected by our use of electrophysiological recording, functional anatomical mapping, advanced cellular imaging methods and behavioral testing in our research. Various combination of these techniques have been used to study cell lines transfected with opioid receptors, cultured sensory neurons, spinal cord and brain slices, and for whole-animal studies. 

Project

• Central hypersensitivity after spinal cord injury 


• Reward, motivation and pain 


• Cellular mechanisms of opioid tolerance 


• Estrogens and inflammation in somatosensory nociceptors
  

 

Grants/Awards

• NSW Ministry of Science and Medical Research: Spinal Cord Injuries and Other Neurological Conditions Program Grant—Keast, Christie, Cousins, Osborne, Siddall “Pain following spinal cord injury: understanding mechanisms to develop treatments”. 


• NIH (USA): NIDDK—Keast, Osborne, Birder “Effects of estrogens on bladder pain”.


• Australian and New Zealand College of Anaesthetists (ANZCA)—Cousins, Osborne “Experimental strategies for preventing persistent postsurgical pain”. 

 

Publications

Marchant, N.J., Densmore, V.S., Osborne, P.B. (2007). Coexpression of prodynorphin and corticotrophin-releasing hormone in the rat central amygdala: evidence of two distinct endogenous opioid systems in the lateral division. J Comp Neurol. 504(6):702-15.

Kalous, A., Osborne, P.B., Keast, J.R. (2007). Acute and chronic changes in dorsal horn innervation by primary afferents and descending supraspinal pathways after spinal cord injury. J Comp Neurol. 504(3):238-53.

Hamlin, A.S., McNally, G.P., Osborne, P.B. (2007). Induction of c-Fos and zif268 in the nociceptive amygdala parallel abstinence hyperalgesia in rats briefly exposed to morphine. Neuropharmacology 53(2):330-43.

Christie, M.J.& Osborne, P.B. (2007) Opioid Electrophysiology in PAG. In: Willis, William D., Jr.; Schmidt, Robert F.; Schmidt, Robert F.; Willis, William D., Jr (eds) Encyclopedia of Pain, Springer, Heidelberg pp. 1532-1534.

Chieng, B.C.H., Christie M.J., Osborne, P.B. (2006). Characterization of neurons in the rat central nucleus of the amygdala: cellular physiology, morphology, and opioid sensitivity. J Comp Neurol. 497(6):910-27.

Osborne, P.B., Halliday, G.M., Cooper, H.M., Keast, J.R. (2005). Localization of immunoreactivity for DCC, the receptor for the guidance factor netrin-1, in ventral tier dopamine projection pathways in adult rodents. Neuroscience, 131, 671-681.

Buller, K.M., Hamlin, A.S., Osborne, P.B. (2005). Dissection of peripheral and central endogenous opioid modulation of systemic interleukin-1beta responses using c-fos expression in the rat brain. Neuropharmacology, 49, 230-242.

Hamlin, A.S., Buller, K.M., Day, T.A., Osborne, P.B. (2004). Effect of naloxone-precipitated morphine withdrawal on c-fos expression in rat corticotropin-releasing hormone neurons in the paraventricular hypothalamus and extended amygdala. Neuroscience Letters, 362, 39-43.

Connor, M., Osborne, P.B., Christie, M.J. (2004). Mu-opioid receptor desensitization: Is morphine different? British Journal of Pharmacology, 143, 685-696.

Bengtson, C.P., Lee, D.J., Osborne, P.B. (2004). Opposing electrophysiological actions of 5-HT on non-cholinergic and cholinergic neurons in the rat ventral pallidum in vitro. Journal of Neurophysiology, 92, 433-443.

Kanjhan, R., Osborne, P.B., Ouyang, M., Keast, J.R. (2003). Postnatal maturational changes in rat pelvic autonomic ganglion cells: a mixture of steroid-dependent and -independent effects. Journal of Neurophysiology, 89, 315-323.

Borgland, S.L., Connor, M., Osborne, P.B., Furness, J.B., Christie, M.J. (2003). Opioid Agonists Have Different Efficacy Profiles for G Protein Activation, Rapid Desensitization, and Endocytosis of Mu-opioid Receptors. Journal of Biological Chemistry, 278, 18776-18784.

Osborne, P.B., Vidovic, M., Chieng, B., Hill, C.E., Christie, M.J. (2002). Expression of mRNA and functional alpha(1)-adrenoceptors that suppress the GIRK conductance in adult rat locus coeruleus neurons. British Journal of Pharmacology, 135, 226-232.

Hamlin, A., Buller, K.M., Day, T.A., Osborne, P.B. (2001). Peripheral withdrawal recruits distinct central nuclei in morphine-dependent rats. Neuropharmacology, 41, 574-581.

Bengtson, C.P., Osborne, P.B. (2000). Electrophysiological properties of cholinergic and noncholinergic neurons in the ventral pallidal region of the nucleus basalis in rat brain slices. Journal of Neurophysiology, 83, 2649-2660.

Osborne, P.B., Chieng, B., Christie, M.J. (2000). Morphine-6b-glucuronide has a higher efficacy than morphine as a mu-opioid receptor agonist in the rat locus coeruleus. British Journal of Pharmacology, 131, 1422-1428.

Bengtson, C.P., Osborne, P.B. (1999). Electrophysiological properties of anatomically identified ventral pallidal neurons in rat brain slices. Annals of the New York Academy of Sciences, 877, 691-694.

Christie, M.J., Williams, J.T., Osborne, P.B., Bellchambers, C.E. (1997). Where is the locus in opioid withdrawal? Trends in Pharmacological Sciences, 18, 134-140.

Osborne, P.B., Williams, J.T. (1996). Forskolin enhancement of opioid currents in rat locus coeruleus neurons. Journal of Neurophysiology, 76, 1559-1565.

Osborne, P.B., Vaughan, C.W., Wilson, H.I., Christie, M.J. (1996). Opioid inhibition of rat periaqueductal grey neurones with identified projections to rostral ventromedial medulla in vitro. Journal of Physiology, 490, 383-389.

Osborne, P.B., Christie, M.J. (1996). Tetrahydro-9-aminoacridine has mixed actions on muscarinic currents and blocks opioid currents in rat locus ceruleus neurons. Journal of Pharmacology & Experimental Therapeutics, 276, 137-142.

Osborne, P.B., Williams, J.T. (1995). Characterization of acute homologous desensitization of mu-opioid receptor-induced currents in locus coeruleus neurones. British Journal of Pharmacology, 115, 925-932.

Davies, P.J., Osborne, P.B., Campbell, G. (1993). The distribution and colocalization of neuropeptides and 5-hydroxytryptamine in pelvic nerves supplying the posterior large intestine of the toad, Bufo marinus. Cell and Tissue Research, 274, 105-114.

Cai, Y.C., Osborne, P.B., North, R.A., Dooley, D.C., Douglass, J. (1992). Characterization and functional expression of genomic DNA encoding the human lymphocyte type n potassium channel. DNA & Cell Biology, 11, 163-172.

Kavanaugh, M.P., Varnum, M.D., Osborne, P.B., Christie, M.J., Busch, A.E., Adelman, J.P., North, R.A. (1991a). Interaction between tetraethylammonium and amino acid residues in the pore of cloned voltage-dependent potassium channels. Journal of Biological Chemistry, 266, 7583-7587.

Kavanaugh, M.P., Christie, M.J., Osborne, P.B., Busch, A.E., Shen, K.Z., Wu, Y.N., Seeburg, P.H., Adelman, J.P., North, R.A. (1991b). Transmitter regulation of voltage-dependent K+ channels expressed in Xenopus oocytes. Biochemical Journal, 277, 899-902.

Hurst, R.S., Busch, A.E., Kavanaugh, M.P., Osborne, P.B., North, R.A., Adelman, J.P. (1991). Identification of amino acid residues involved in dendrotoxin block of rat voltage-dependent potassium channels. Molecular Pharmacology, 40, 572-576.

Pan, Z.Z., Williams, J.T., Osborne, P.B. (1990). Opioid actions on single nucleus raphe magnus neurons from rat and guinea-pig in vitro. J Physiol Lond, 427, 519-532.

Murphy, S., Osborne, P.B., Adamson, S., Campbell, G. (1990). Co-localization of calcitonin gene-related peptide- and substance P-like immunoreactivity in mucosal intra-epithelial nerves in the toad colon. Neuroscience Letters, 116, 7-11.

Douglass, J., Osborne, P.B., Cai, Y.C., Wilkinson, M., Christie, M.J., Adelman, J.P. (1990). Characterization and functional expression of a rat genomic DNA clone encoding a lymphocyte potassium channel. Journal of Immunology, 144, 4841-4850.

Christie, M.J., North, R.A., Osborne, P.B., Douglass, J., Adelman, J.P. (1990). Heteropolymeric potassium channels expressed in Xenopus oocytes from cloned subunits. Neuron, 4, 405-411.

Osborne, P.B., Campbell, G., Evans, B.K. (1989). Distribution of substance P in the enteric plexuses of the small intestine of the platypus, Ornithorhynchus anatinus. Cell and Tissue Research, 255, 663-667.

Morris, J.L., Gibbins, I.L., Osborne, P.B. (1989). Galanin-like immunoreactivity in sympathetic and parasympathetic neurons of the toad Bufo marinus. Neuroscience Letters, 102, 142-148.

Osborne, P., Campbell, G. (1986). A pharmacological and immunohistochemical study of the splanchnic innervation of ileal longitudinal muscle in the toad Bufo marinus. Naunyn Schmiedebergs Arch Pharmacol, 334, 210-217.

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